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. 2012 Sep 1;26(17):1897–1910. doi: 10.1101/gad.197467.112

Figure 6.

Figure 6.

The CAB boxes of AluACA RNAs function in Wdr79 binding. (A) The proximal and distal CAB boxes of AluACA7 bind Wdr79 in a cumulative manner. Nucleotide alterations introduced into the proximal and distal CAB box of AluACA7 are indicated. Extracts prepared from HeLa cells transfected with the pGL/ALU7 (WT), pGL/ALU7pCAB, pGL/ALU7dCAB, or pGL/ALU7pdCAB expression plasmids were reacted with an anti-Wdr79 antibody. RNAs isolated from cell extracts and the pellets of IP reactions were analyzed by RNase mappings. (B) The CAB boxes of AluACA RNAs are essential for both Wdr79 binding and efficient RNA accumulation. Schematic structure of the U64-AluACA7 composite RNA is shown. Extracts prepared from HeLa cells nontransfected (NT) or transfected with pGL/U64-ALU7 (WT) or pGL/U64-ALU7pdCAB were reacted with an anti-Wdr79 antibody. RNAs isolated from cell extracts and the pellets of IP reactions were analyzed by RNase protection. The first exon (E1) of globin mRNA is indicated.