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. 2012 Sep 1;26(17):1945–1958. doi: 10.1101/gad.193458.112

Figure 3.

Figure 3.

Analysis of L597VBraf-expressing lung. (A) H&E-stained lung sections taken from Braf+/+;CMV-Cre+/o mice, Braf+/Lox-L597V;CMV-Cre+/o mice, or Braf+/Lox-L597V mice lacking the CMV-Cre transgene. Bars, 100 μm. (B) H&E-stained lung sections taken from Braf+/+, Braf+/LSL-L597V, and Braf+/LSL-V600E mice treated with 1 × 108 plaque-forming units (pfu) by nasal inhalation 8 wk post-AdCre infection. Bars, 100 μm. (C) Braf kinase assays of protein lysates prepared from the lungs of Braf+/+;CMV-Cre+/o (wild-type) and Braf+/Lox-L597V;CMV-Cre+/o (LV) mice as well as protein lysates prepared from Braf+/LSL-V600E mice treated with 1 × 108 pfu of AdCre by nasal inhalation 8 wk post-AdCre infection (VE). Columns indicate the mean of three technical replicates of three different biological samples, and bars indicate the SD. P-values were calculated using the Student's t-test; (**) P < 0.005; (*) P < 0.01. (D) Western blot analysis of protein lysates prepared from the lungs of Braf+/+;CMV-Cre+/o (wild-type), Braf+/Lox-L597V;CMV-Cre+/o (LV), and Braf+/LSL-V600E mice treated with 1 × 108 pfu of AdCre by nasal inhalation 8 wk post-AdCre infection (VE). Western blots were analyzed with the antibodies indicated.