Figure 7.

Phosphatase hits affect cell-cycle timing. In HeLa cells, V5- or GFP-tagged protein phosphatases or GFP coding vector, as negative control, were overexpressed. After 24 h of transfection, cells expressing V5-tagged phosphatases were fixed, stained with anti-Ki67 and anti-V5 antibodies and analyzed by immunofluorescence microscopy coupled to automated image analysis. The percentage of transfected cells having Ki67-positive nuclei was measured. Two independent biological replicas were plotted in the bar graph.