Fig. 1.

Plasma decay, liver uptake, and distribution of radiolabeled cholesterol from chylomicron-like emulsion particles in WT mice. After tail vein injection of emulsion particles containing [¹⁴C]cholesterol and [³H]CO into WT mice (n = 5), plasma samples were obtained at the indicated times and plasma decay was determined (A). After intestine perfusions, the remaining activity of both radiolabels in the liver was determined as % of injected dose (B). Liver samples were analyzed for distribution of ¹⁴C and ³H over free (FC) and esterified cholesterol (CE) via TLC (C). Lipoproteins in pooled plasma samples obtained at 2.5 h after injection were separated by FPLC and in the obtained fractions both total plasma cholesterol and radioactivity were determined (D). Free cholesterol and cholesteryl esters in plasma were separated by TLC and ¹⁴C and ³H radioactivity was measured in the different fractions (E). Values are means ± SD.