Mitochondrial raft-like microdomains and apoptotic susceptibility. A: Biparametric flow cytometry analysis of apoptosis after double staining of cells with annexin V-FITC/Trypan blue. In the left panel, bar graph shows the results obtained from three independent experiments reported as mean ± SD. * P < 0.01 cells treated with MBC+STS or FB1+ STS versus cells from HS treated with STS; ° P < 0.01 cells treated with MBC+STS or FB1+ STS versus cells from HD patients treated with STS. In the right panels, dot plots from a representative experiment are shown. Numbers represent the percentage of annexin V single-positive cells (early apoptosis, lower right quadrant) or annexin V/Trypan blue double-positive cells (late apoptosis, upper right quadrant). B: Cytofluorimetric analysis of superoxide anion production. Reported values represent the median fluorescence. In the left panel, bar graph shows the results obtained from three independent experiments reported as mean ± SD. ° P < 0.01 cells treated with MBC+STS versus cells from HD patients treated with STS; ° P < 0.01 cells treated with FB1+STS versus cells from HD patients treated with STS. In the right panels, results obtained in a representative experiment are shown as fluorescence emission histograms. Dotted lines in left panels indicate values found in HS cells treated with STS, whereas dashed lines indicate values found in HD cells treated with STS (corresponding to data reported in Fig. 1). C: Biparametric flow cytometry analysis of MMP after staining with JC-1. In the left panel, bar graph shows the results obtained from three independent experiments reported as mean ± SD. Full and empty columns represent the percentages of cells with hyperpolarized (MMH) or depolarized (MMD) mitochondrial membrane, respectively. * P < 0.01 cells treated with MBC+STS versus cells from HD patients treated with STS; * P < 0.01 cells treated with FB1+STS versus cells from HD patients treated with STS. In the right panels, dot plots from a representative experiment are shown. Numbers reported in the boxed area represent the percentages of cells with hyperpolarized mitochondria. In the area under the dotted line, the percentage of cells with depolarized mitochondria is reported. Results obtained in a representative experiment are shown.