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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Free Radic Biol Med. 2012 Jul 13;53(5):1048–1060. doi: 10.1016/j.freeradbiomed.2012.07.004

Figure 8. Increased levels of phosphorylation of 4E-BP1 due to endogenous oxidative stress in synaptosomes.

Figure 8

(A) levels of phosphorylated mTOR do not change as a consequence of endogenous oxidative stress. However, levels of phosphorylated 4E-BP1 are significantly increased in synaptosomes from (p=0.0023) Sod2 null mice. (B). Levels of ULK1, a protein involved in autophagy are not changed in synaptosomes from Sod2 null mice. Equivalent amounts of synaptosomal proteins between wild-type controls and Sod2 null mice (SNAP25 synaptosomal marker) were loaded, and then levels of ULK1 (Unc 51 kinase like 1, a key protein involved in autophagy mediated through TOR) were estimated. No significant differences were seen in synaptosomal levels of ULK1.