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. 2012 Jul 5;287(35):29457–29467. doi: 10.1074/jbc.M112.365676

FIGURE 3.

FIGURE 3.

atg-4.1 and atg-4.2 function redundantly in mediating conjugation of LGG-1. A, LGG-1/Atg8 levels in atg-4.1 and atg-4.2 mutants. Western analysis shows accumulation of LGG-1 precursors and also LGG-1-II in atg-4.1 mutants. LGG-1-I is very weakly detected in bp321, bp451, and bp504 mutants and is absent in bp501 mutants. In atg-4.2 mutants, no LGG-1 precursors are detected. LGG-1-II accumulates at a higher level than in wild type animals. B–E, atg-4.1- and atg-4.2-null mutants exhibit the wild-type dynamic immunostaining pattern of LGG-1 during embryogenesis. B, DAPI image of the embryo in C. F, in atg-4.1(bp501); atg-4.2(tm3948) double mutants, SEPA-1 aggregates accumulate at the comma stage. G, no LGG-1 puncta are detected in atg-4.1(bp501); atg-4.2(tm3948) double mutants.