FIGURE 5.

Expression of the C. jejuni N-linked glycosylation system, EptC-HA, and AcrA-His in E. coli. A, upper, induction (I) of EptC with arabinose from pNS3 compared with non-induced controls (NI) and empty vector control pMLBAD is shown. Lower, comparison of AcrA glycosylation (0G, 1G, and 2G refer to the number of occupied N-glycosites). B, XIC of pEtN- glycan-modified (i and iii), E. coli heptasaccharide-modified (i and ii) and canonical C. jejuni N- glycan-modified (iii and iv) glycopeptides from AcrA ¹¹³ATFENASKDFNR¹²⁴ are shown. C, confirmation of glycan structures by CID MS/MS (i–iv).