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. 2012 Jun 21;287(35):29702–29712. doi: 10.1074/jbc.M112.367706

FIGURE 2.

FIGURE 2.

Immunoreactivity of NDAT with Thr(P)53 Ab. A, rat DAT N-terminal amino acid sequence (residues 1–65; NDAT) highlighting the PKC-dependent phosphorylation domain (PKC) and the ERK proline-dependent phosphorylation site (ERK). B, NDAT samples given no kinase treatments or phosphorylated in vitro by PKCα or ERK1 were immunoblotted with Thr(P)53 Ab (pT53Ab) (top) or mAb 16 (bottom). Where indicated, Thr(P)53 Ab was preincubated with phosphorylated (pT53) or non-phosphorylated (Ctl) N-terminal peptide. Molecular mass markers are indicated on the right.