FIGURE 5.

Expression and kinetic analysis of T53A and T53D DATs. rDAT-LLCPK₁ cells stably expressing WT, T53A, or T53D DATs were assayed in parallel for total DAT expression levels (A), surface DAT expression levels (B), and DA uptake saturation analysis (C). Blots shown are representative of four independent experiments with samples run in duplicate. Histograms show quantification of DAT levels (**, p < 0.01; *, p < 0.05 relative to controls by ANOVA with Tukey's post hoc test). Transport kinetic parameters were determined by nonlinear regression analysis in three or four independent experiments performed in triplicate. Data are presented as means ± S.E. normalized to cell surface DAT expression levels (**, p < 0.01; *, p < 0.05 relative to controls by ANOVA with Tukey's post hoc test).