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. 2012 Jul 10;287(35):29636–29647. doi: 10.1074/jbc.M112.344192

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — The inactivation of the intra-S checkpoint after caspase-10 expression is dependent on Far11. A, scheme of the regulation of the intra-S checkpoint. B, yeast strains BY4741/pESC-URA/CASP10, far11Δ/pESC-URA/CASP10, and far11Δ/pESC-URA/CASP10/YCplac111/FAR11 were grown in galactose-containing medium, and the DNA content was analyzed by FACS in both asynchronous and HU-treated cultures. The predicted phosphorylation state of Rad53 is indicated. C, Western blot of protein extracts from the yeast strains BY4741 and far11Δ transformed with either pESC-URA/CASP10 or the empty vector. Cells were grown in galactose-containing medium and treated with HU. The blot was probed with an anti-Rad53 antibody (yC-19). Ponceau red staining of the blot was used as a protein loading control.