FIGURE 3.

SPDEF knockdown in LNCaP-Luc cells increases tumor metastasis. A and B, representative images of bioluminescent images of mice injected with Scr-Luc (n = 7) and SPDEF KD-Luc (n = 5) cells (n represents each model), representative mice are shown. Shown are images at week 1 and week 12 post injection. C and D, quantitation of luciferase signal in the mice at the various time points shown. A significant increase in disseminated tumor cells (luciferase activity) were detected in the SPDEF KD-Luc cells compared with the Scr-Luc cells at 12 weeks. Scr-Luc, Scr-LNCaP-Luc; SPDEF KD-Luc, SPDEF-KD-LNCaP-Luc. *, statistical significance (p < 0.05) compared with Scr-Luc cells at 12 weeks.