FIGURE 5.

SPDEF expression has no effect on subcutaneous tumor xenograft growth. A and B, nude mice (n = 5 per group) were subcutaneously injected with either VC-Luc or SPDEF OE-Luc cells. Tumors growth was followed for 42 days, and tumor volume was measured. A, representative image of xenografts using in vivo bioluminescent imaging. B, quantitation of tumor volumes over the experimental period. C, at the end of the experimental period, tumors were dissected and weighed. D, Western blot analysis of cell cycle regulatory proteins. Note that SPDEF expression was increased and was used as an internal positive control. E, immunohistochemistry of the cell cycle inhibitors p21 and p27. F, RT-PCR analysis of expression of cell cycle regulatory genes. Graphs represent densitometry of three independent experiments of the RT-PCR data compared with GAPDH control. VC-Luc, VC-PC3-Luc; SPDEF OE-Luc, SPDEF-PC3-Luc. *, statistical significance (p < 0.05) compared with VC-Luc cells.