TABLE 1.
RT-PCR primer sequences
| Gene | RT-PCR primer sequences |
|||
|---|---|---|---|---|
| Forward | Temperature (°C) | Reverse | Temperature (°C) | |
| Cyclin B | 5′-TTGATACTGCCTCTCCAAGCCCAA-3′ | 60.3 | 5′-TTGGTCTGACTGCTTGCTCTTCCT-3′ | 60.3 |
| Cyclin D | 5′-CCTTTGGTGCCAACTGGTGTTTGA-3′ | 60.3 | 5′-TCAGATGACTCTGGGAAACGCCAA-3′ | 60.3 |
| Cyclin E | 5′-TGCAGAGCTGTTGGATCTCTGTGT-3′ | 60.3 | 5′-ACAACATGGCTTTCTTTGCGCGGG-3′ | 60.3 |
| CDK2 | 5′-AGCCAGAAACAAGTTGACGGGAGA-3′ | 60.5 | 5′-AAGAGGAATGCCAGTGAGAGCAGA-3′ | 60.0 |
| p27 | 5′-AGTCCATTTGATCAGCGGAGACTCG-3′ | 60.4 | 5′-TCGCACGTTTGACATCTTTCTCCC-3′ | 59.5 |
| p21 | 5′-TTCGACTTTGTCACCGAGACACCA-3′ | 60.2 | 5′-AGGCACAAGGGTACAAGACAGTGA-3′ | 60.1 |
| VASP | 5′-GTAAGAGTAACACTGTAGCCGCCA-3′ | 58.5 | 5′-ATCATAAAGCATCACAGTGGCCCG-3′ | 59.6 |
| Survivin | 5′-GAGGCTGGCTTCATCCACTG-3′ | 58.2 | 5′-CAGCTGCTCGATGGCACGGC-3′ | 64.1 |
| Snail | 5′-TGCCAATGCTCATCTGGGACTCT-3′ | 60.5 | 5′-GCCTCCAAGGAAGAGACTGAAGTA-3′ | 57.9 |
| MMP9 | 5′-TACCACCTCGAACTTTGACAGCGA-3′ | 60.1 | 5′-AAAGGCACAGTAGTGGCCGTAGAA-3′ | 60.3 |
| Slug | 5′-ACTACAGTCCAAGCTTTCAGACCC-3′ | 58.6 | 5′-CCGCAGATCTTGCAAACACAAGGT-3′ | 60.2 |
| MMP13 | 5′-AACATCCAAAAACGCCAGAC-3′ | 53.9 | 5′-GGAAGTTCTGGCCAAAATGA-3′ | 53.3 |
| SPDEF | 5′-CAGGTGAAGTCCGCTCTTTC-3′ | 55.7 | 5′-AATGTGCAGAAGTGGCTCCT-3′ | 56.9 |
| IL-6 | 5′-TACCCCCAGGAGAAGATTCC-3′ | 55.7 | 5′-AAAGCTGCGCAGAATGAGAT-3′ | 55.0 |
| CXCR4 | 5′-TGGCCTTATCCTGCCTGGTATTGT-3′ | 60.2 | 5′-TGGCTCCAAGGAAAGCATAGAGGA-3′ | 59.9 |
| GAPDH | 5′-AAGGTCGGAGTCAACGGATTTGGT-3′ | 60.5 | 5′-AGTGATGGCATGGACTGTGGTCAT-3′ | 60.5 |