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. 2012 Jul 6;287(35):30000–30013. doi: 10.1074/jbc.M112.359125

FIGURE 5.

FIGURE 5.

Effect of anti-GPVI antibodies on binding of GPVI dimer to collagen, collagen peptides, and representative collagen Toolkit III peptides. ELISA was used to measure GPVI dimer binding to immobilized collagen I and III, CRP, (GPO)2, (GPO)2(GPP)4(GPO)2, and three representative collagen Toolkit III peptides: III-30 (high affinity), III-40 (moderate affinity), and III-39 (low affinity). A fixed concentration of GPVI dimer (20 μg/ml) was preincubated with various concentrations of an antibody before binding to immobilized peptide. Antibodies used were as follows: GPVI dimer-specific antibodies: m-Fab-F (black open circles) and 204-11 Fab (gray closed diamonds); and an antibody binding to both GPVI dimer and monomer, 1C3 (black closed squares). Each point on the curve represents the mean ± S.E. of triplicate determinations. Where no error bars are visible, they are equal to or smaller than the size of the symbol.