FIGURE 5.

Expression of AC isoforms in adult mouse cardiac myocytes. A and B, AC isoform mRNA expression as determined by semiquantitative PCR from total RNA in mouse brain and adult mouse cardiac myocytes, respectively. Lanes 1–9 indicate PCR amplification of AC1–9. DNA markers (M) are indicated. PCR was performed as described under “Experimental Procedures” using the corresponding primer pairs for all mouse transmembrane AC isoforms (AC1–9). Adult mouse cardiac myocytes expressed AC4–6 and AC9.