FIGURE 7.
KCNQ1-KCNE1 associates with AC9 activity in guinea pig heart. A, guinea pig heart extracts were immunoprecipitated (IP) with anti-KCNQ1 or anti-Yotiao antibody or control IgG (goat IgG (IgGg), or rabbit IgG (IgGr)). Immunoprecipitates were stimulated with 400 nm Gαs, and associated AC activity was measured. Data are presented as the mean ± S.E. (n = 3). ***, p < 0.001. B, guinea pig heart extracts were subjected to immunoprecipitation with anti-KCNQ1 antibody and goat IgG or with anti-Yotiao antibody and rabbit IgG. Immunoprecipitates were subjected to Western blot analysis using anti-Yotiao, anti-KCNQ1, and anti-AC9 antibodies.