FIGURE 5.

A, localization of Arc with mutant huntingtin aggregates in HD150Q cells. HD16Q and HD150Q cells were plated onto 2-well tissue culture chamber slides and induced with 1 μm ponasterone A for 48 h. Cells were then processed for immunofluorescence staining using anti-Arc antibody. Rhodamine-conjugated secondary antibody was used to detect Arc. Arrows indicate the localization of Arc in the huntingtin aggregates. Scale bar = 20 μm. B, Arc levels are increased in HD150Q cells. HD16Q and HD150Q cells were left uninduced or induced with ponasterone A for 48 h, and the cell lysates were made and subjected to immunoblot analysis using antibodies against Arc, Ube3a, GAPDH, and GFP (to detect 16Q and 150Q proteins).