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. 2012 Jun 26;287(35):29442–29456. doi: 10.1074/jbc.M112.378901

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Ada3 deletion abrogates histone acetylation by destabilizing various HATs. A–C, Western blotting analysis of lysates from asynchronous (A and C) or serum-restimulated (B) Ada3^FL/FL or Ada3^FL/FL/fhAda3 MEFs infected with control (Ctrl) or Cre adenoviruses using the indicated antibodies. D and E, Ada3 enhances p300 HAT activity. In vitro HAT assay using purified recombinant human Ada3 and core histones (D) or histone H3 alone (E) along with their respective Ponceau blots to indicate equal loading is shown.