FIGURE 10.

GR activated PKCϵ also protects against Cisplatin/Irradiation induced apoptosis in MCF10Amyc. MCF10Amyc cells were transfected with nontargeting siRNA (siCon) or PKCϵ-specific siRNA (siPKCϵ). After 24 h, Dex (1 μm) was added for 30 min before treating. A, Cis (Cis; 5, 15 μm); B, irradiation (IR; 4, 10 Gy) in −ALL condition. After 48 h post-treatment, cells were harvested and stained with propidium iodide to measure DNA content. C and D, cell lysates were collected and analyzed by immunoblot analysis with indicated antibodies. The analysis was done in duplicate assays, and data are presented in C as means ± S.E. (n = 2).