Skip to main content
. 2012 Jul 12;287(35):29373–29383. doi: 10.1074/jbc.M112.354001

FIGURE 1.

FIGURE 1.

The 7SL RNA mediates the interaction between A3G and MOV10. A, 293T cells transfected with A3G-HA- (or A3G-W127L-HA-) and MOV10-FLAG-expressing plasmids were collected and lysed. B, 293T cells transfected with A3G-HA- (or A3G-W127L-HA-) expressing plasmids were collected and lysed. A and B, after immunoprecipitation with anti-HA agarose beads, the samples were treated with or without RNase mixture and then analyzed by immunoblotting using anti-FLAG (A), anti-MOV10 (B), or anti-HA antibody (A and B). C, SRP14 mRNA in 293T cells expressing control-shRNA and SRP14-shRNA was quantified using real-time RT-PCR at 72 h post-transfection. The SRP14 mRNA in cells expressing control-shRNA was defined as 100%. D, 7SL RNA in 293T cells expressing control-shRNA and SRP14-shRNA was quantified by real-time RT-PCR at 6 days post-transfection. The 7SL RNA in cells expressing control-shRNA was defined as 100%. Data in C and D represent mean ± S.D. E, 293T cells expressing control-shRNA or SRP14-shRNA were co-transfected with MOV10-FLAG- and A3G-HA- (or A3G-W127L-HA-) expressing plasmids. F, 293T cells expressing control-shRNA or SRP14-shRNA were transfected with A3G-HA- (or A3G-W127L-HA-) expressing plasmids. E and F, after 24 h, cells were lysed and co-immunoprecipitated with anti-HA agarose beads. The precipitated samples were analyzed by immunoblotting using anti-FLAG (E), anti-MOV10 (F) or anti-HA antibody (E and F). Values in A, B, E, and F represent portions of MOV10-FLAG (or MOV10) normalized against A3G-HA (or A3G-W127L-HA) relative to control values.