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. 2012 Jul 12;287(35):29373–29383. doi: 10.1074/jbc.M112.354001

FIGURE 6.

FIGURE 6.

A3G and MOV10 do not affect tethered AGO1- or AGO2-mediated down-regulation of protein synthesis. A, scheme of the tethering assay system. psi-5BoxB Renilla mRNA, containing five 19-nt BoxB hairpins in the 3′-UTR of psiCHECK-2, interacting with λN-AGO1 or λN-AGO2. B, 293T cells were co-transfected with A3G-expressing plasmid, pλN-AGO1 (or pλN-AGO2) and psi-5BoxB. pAGO1 (or pAGO2) and pcDNA3.1 were transfected as controls. At 48 h post-transfection, Renilla/firefly luciferase activity was measured. C, 293T cells were first transfected with MOV10-specific siRNA (gfp-specific siRNA as a control). After 24 h, cells were co-transfected with psi-5BoxB and pλN-AGO1 (or pλN-AGO2). pAGO1 (or pAGO2) was transfected as a control. At 24 h post-transfection, Renilla/firefly luciferase activity was measured. Data in B and C represent mean ± S.D. (error bars). D, competitive inhibition of interaction between AGO2 and MOV10 by A3G.