FIGURE 1.

mAb 4B8 specific to cardiac TnT across vertebrate species. Western blots using 14% SDS-PAGE with acrylamide:bisacrylamide ratio of 180:1 characterized the isoform specificity of anti-TnT mAb 4B8 generated against human cardiac TnT immunogen. mAb 4B8 recognized TnT in adult cardiac but not skeletal muscles from all mammalian, avian, amphibian, and fish species examined except for one variant in fish red muscle. The cardiac TnT bands recognized by mAb 4B8 were confirmed in Western blots using mAb CT3 that recognizes both cardiac TnT and ssTnT and blots using mAb 2C8 that recognizes all three muscled type isoforms of TnT across vertebrate species. Western blot using mAb TnI-1 detected cardiac TnI, slow skeletal muscle TnI, and fast skeletal muscle TnI in the muscle samples, verifying the presence of all three fiber types. The results demonstrate that mAb 4B8 is a highly valuable cardiac TnT-specific antibody and can be used for studies in all vertebrate species.