FIGURE 5.
Phylogenetic and sequence studies of toad ssTnT. A, the phylogenetic tree was constructed from aligning amino acid sequences of vertebrate cardiac TnT and ssTnT isoforms using DNA Star Clustal W software. The result demonstrated a conserved sequence of toad ssTnT, which is distinct from cardiac TnT (cTnT). B, alignment of amino acid sequences of toad ssTnT isoforms, Xenopus ssTnT, and Xenopus cardiac TnT. The results showed that toad ssTnT is similar to Xenopus ssTnT but significantly different from cardiac TnT in the NH2-terminal variable region and a lack of six amino acids near the COOH terminus. GenBank accession numbers: Xenopus ssTnT, NM_001092738.1; Xenopus cardiac TnT high molecular weight (MW) isoform, AF467919.1; Xenopus cardiac TnT low Mr isoform, AF467920.1.