Table I.
Antibodies and protocol of immunohistochemical reaction.
| Antibody (clone) | Dilution | Antigen retrieval | Secondary antibody |
|---|---|---|---|
| Anti-CD4a (0.N.52) | 1:100 | EDTA buffer (pH 8.0 for 30 min at 98°C) | EnVision |
| Anti-CD25a (N-19) | 1:50 | EDTA buffer (pH 8.0 for 30 min at 98°C) | EnVision |
| Anti-CTLA-4a (C-19) | 1:1200 | Citrate buffer (pH 6.0 for 30 min at 95°C) | Kit-LSAB |
| Anti-FoxP3a (236A/E7) | 1:400 | Citrate buffer (pH 6.0 for 30 min at 95°C) | Kit-LSAB |
| Ki67b (MM1) | 1:100 | Citrate buffer (pH 6.0 for 30 min at 95°C) | Kit-LSAB |
a
From Santa Cruz Biotechnology (Santa Cruz, CA, USA);
b
From Novocastra (Newcastle, UK).