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Aloe-emodin induces the release of apoptosis-inducing factor and cytochrome c (cyt c) in DLD-1 and WiDr cells. Cells were incubated with or without 0.37 mM aloe-emodin for 0, 3, 6, 12 and 24 h. Mitochondrial (mito) and cytosolic (cyto) fractions were analyzed by 15% SDS-PAGE and probed with primary antibodies as described in Materials and methods. Results are representative of three independent experiments.
