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Specificity of AS-LAMP on cell lines. Eight JAK2 wild-type (GFD8, HL60, K562, TF1, NB4, KASU-MI-1, U266B1, BJAB) and three JAK2V617F mutant cell lines (HEL, SET-2 and UKE-1) were analyzed by AS-LAMP. Within 60 min, only DNA samples obtained from mutant cell lines showed an amplified product, while no amplification was detected in any of the wild-type samples.
