Figure 4.

Allele burden determination. (A) A standard curve of the AS-LAMP assay was created using three calibrators (1%-10%-100% JAK2V617F allele) and the indicated DNA dilutions of UKE-1 into BJAB (from 90% to 2%) were tested. DNA samples containing an allele burden of 2% and 5% could be directly defined as low positive (allele burden between 1% and 10%). DNA samples containing a JAK2V617F allele burden between 20% and 90% were appropriately defined as having an allele burden between 10% and 100%. (B) A further 1:5 dilution of these latter samples allowed discrimination of samples into those with a JAK2V617F allele burden above or below 50% (see text).