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(A) Genomic quantitative-PCR for gene dosage analysis to calculate the copy numbers of γ-, δ- and β-globin genes. The peak heights of amplified products of the globin genes and the control albumin gene are shown. (B) MLPA analysis of the β-globin cluster in the family. Arrows indicate the genomic regions that are deleted. Father is heterozygous for an additional deletion of Gγ-globin.
