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Bcl-2 expression correlates with the miR-16 level. A cell-free extract was subjected to Western blotting with the Bcl-2 antibody 48 h post-transfection. Lane 1, mimic negative control; lane 2, miR-16 mimic oligonucleotide; lane 3, inhibitor negative control; lane 4, miR-16 inhibitor.
