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. 2010 May 1;1(3):565–568. doi: 10.3892/ol_00000100

Figure 3.

Figure 3

Direct identification of BCL2 3′ UTR by miR-16 in pancreatic cancer cells. (A and B) 3′ UTR (220 bp) of the BCL2 gene cloned in pGL3 control vectors in both sense (pGL3 BCL2) and antisense (pGL3 BCL2 AS) orientations. For luciferase reporter assay, human pancreatic cancer BxPC-3 cells were co-transfected in a 96-well plate with 100 ng of firefly luciferase reporter vector (pGL3-BCL2 UTR sense and antisense) and 10 ng of the pEGFPC2 expression vector in the presence of 50 nM miR-16 mimic oligo or 50 nM mimic negative control. The luciferase activity was measured (Allele Biotechnology, San Diego, CA, USA), 48 h post-transfection, and normalized to GFP expression. Notably, the luciferase activities of the control plasmids were considered to be 1.