Table I.
Evaluation of secreted analytes.a
| Analyte | 435- | 10% FBS (mean ± range) | 1% FBS (mean ± range) |
|---|---|---|---|
| MMP-3 (ng/ml) | X7 | 34.4±13.3 | 12.8±4.8 |
| WT | 2.8±0.6 | 0.6±0.0 | |
| Compl. C3 (ng/ml) | X7 | 6.7±1.0 | 3.0±0.0 |
| WT | 0.7±0.1 | 0.7±0.3 | |
| CCL5 (pg/ml) | X7 | 323.5±13.5 | 206.0±24.0 |
| WT | 56.5±15.7 | 43.3±14.6 | |
| TIMP-1 (ng/ml) | X7 | 205.6±194.5 | 125.7±111.4 |
| WT | 114.7±102.4 | 67.8±53.3 | |
| GM-CSF (pg/ml) | X7 | 16.2±2.7 | 28.7±7.6 |
| WT | 11.6±0.6 | 7.2±0.2 | |
| CCL2 (pg/ml) | X7 | 3145±375 | 1500±40 |
| WT | 2410±230 | 851±54 |
a
Multiplex immunoassay of the conditioned media from 1 million 435-WT or 435-CXCR7 cells cultured for 48 h with 10 or 1% FBS.
FBS, fetal bovine serum.