FIGURE 10.

ΔNp63α affects cell shape and motility. a, SCC011 cells were transiently silenced with IBONI p63-siRNA pool (20 nm final concentration). Cells were fixed and subjected to double immunofluorescence 48 h after silencing, as already described. b, protein extracts from control and p63-depleted cells were separated by SDS-PAGE and subjected to immunoblot. Proteins were detected with specific antibodies. c, actin staining with TRITC-conjugated phalloidin of control and ΔNp63α-depleted SCC011 cells (see “Experimental Procedures”). Cell expressing ΔNp63α displayed a round morphology. Conversely, ΔNp63α-depleted cells had a polarized morphology with more evident lamellipodia and trailing edge. d, windrose plot of trajectories described by ΔNp63α depleted SCC011 or SCC011 control cells (inset) in 12-h time lapse video. Only 15 cell trajectories were reported in each plot for graphical clarity. E-cad, E-cadherin; N-cad, N-cadherin.