FIGURE 3.

Three-dimensional localization of RyR1-bound Ca²⁺-CaM_WT. A and B, three-dimensional reconstruction of Ca²⁺-CaM_WT-RyR1. A shows a side view, and B shows a 4-fold view of the bottom (membrane-facing) surface. C and D, side view and bottom 4-fold view, respectively, of the CaM-free RyR1 control. Red circled regions in A–D highlight the major differences between Ca²⁺-CaM_WT-RyR1 and the CaM-free RyR1 control. E and F, densities likely due to RyR1-bound Ca²⁺-CaM_WT that were generated by volume subtraction of the RyR1 control from Ca²⁺-CaM_WT-RyR1. Scale bar = 10 nm.