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. 2012 Jul 9;287(36):30336–30345. doi: 10.1074/jbc.M111.329110

FIGURE 1.

FIGURE 1.

Expression of BMPER in murine tissues and cultured human cells. A, mRNA expression of BMPER and GAPDH in murine tissues. B, mRNA expression of BMPER and GAPDH in cultured human cells. HAoEC, human aortic endothelial cell (EC); HCAEC, human coronary artery EC; HUVEC, human umbilical vascular EC; HMVEC, human lung microvascular EC; HAoSMC, human aortic SMC; HPASMC, human pulmonary artery SMC; HEK293, human embryonic kidney 293 cell. C, expression and secretion of BMPER by HCASMCs. The conditioned media of HCASMCs cultured for the indicated time periods were subjected to Western blotting. The leftmost lane was subjected with cell-free media. D, mRNA expression of BMPER in HCASMCs and HUVECs treated with TNF-α (10 ng/ml) for the indicated time periods. Each value was standardized by the GAPDH mRNA expression and is presented as the fold increase over that in the control at day 0 (n = 3). E–H, subcellular localization of BMPER in HCASMCs. HCASMCs were doubly stained with an anti-BMPER pAb and an anti-GM130 mAb (E), an anti-clathrin mAb (F), an anti-EEA1 mAb (G), or an anti-mitochondrial Hsp70 mAb (H). The nuclei were stained with DAPI.