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. 2012 Jul 13;287(36):30368–30375. doi: 10.1074/jbc.M112.375618

FIGURE 4.

FIGURE 4.

BRSK2 inhibits glucose-stimulated insulin secretion in MIN6 cells. A, insulin secretion in BRSK2 overexpression or BRSK2-RNAi depletion MIN6 cells incubated in 3 mm glucose or 25 mm glucose, respectively. ***, p < 0.001, n = 6/condition. B, insulin secretion in PCTAIRE1 overexpression or PCTAIRE1-RNAi deletion MIN6 cells incubated in 3 mm glucose or 25 mm glucose, respectively. Overexpressing PCTAIRE1 could partly reverse the BRSK2-depletion influence on insulin secretion, while overexpressing BRSK2 had no effect on PCTAIRE1-depletion cells. ***, p < 0.001, n = 6/condition. The transient transfection was performed for 48 h and its expression in MIN6 cells was analyzed by Western blot using Myc, BRSK2, or PCTAIRE1 antibody. PCMV-Myc-null vector and a nonsilence RNAi construct are used as a control for overexpression or RNAi, respectively.