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Na+-dependent modulation of probe binding to mIIaQQQ. Stopped-flow fluorescence traces of the binding of I-2581 to mIIaQQQ at final concentrations of 0.5 and 0.3 μm, respectively, are illustrated at final Na+ concentrations ranging from 0 to 150 mm (bottom to top). Inset, fractional amplitudes of the slow phase (○) and the fast phase (●) as a function of Na+.
