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Reaction of mIIa with an active site probe (P). mIIa is proposed to exist in an equilibrium between e and E. E binds P with high affinity to form E·P, whereas e binds P with much weaker affinity. The fluorescence increase associated with the formation of e·P or E·P is assumed to be identical. The interconversion between e·P and E·P is described by KConf = e·P/E·P.
