FIGURE 1.

DSPP mRNA levels in the incisor of the Dspp-KO/D452A-Tg and Dspp-KO/normal-Tg mice. RNA isolated from the incisor of 1-month-old mice was used for real-time PCR analyses. The mRNA level in the WT mouse incisor was taken as one, while that of the Dspp-KO/normal-Tg or Dspp-KO/D452A-Tg mice was expressed as fold over the WT mice. The level of the transgenic DSPP mRNA in the Dspp-KO/normal-Tg mice was ∼16-fold of that of the endogenous Dspp gene in the WT mice. The level of mRNA from the D452A-DSPP transgene in the Dspp-KO/D452A-Tg mice was ∼13-fold of that of the endogenous Dspp gene in the WT mice. The forward primer sequence used for real-time PCR analysis was from exon 3 of the endogenous Dspp gene, while the reverse was from exon 4. The results were from five analyses (n = 5) for each group.