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Anti-DSP immunohistochemistry. The specimens were from the first mandibular molars of four types of mice at postnatal 3 months. The samples from the Dspp-KO mice (B) were used as negative controls. Anti-DSP activity was observed in the dentin matrix of the WT (A), Dspp-KO/normal-Tg (C), and Dspp-KO/D452A-Tg (D) mice. The signal for the anti-DSP antibody in the dentin of the Dspp-KO/D452A-Tg mice was weaker than in the WT or Dspp-KO/normal-Tg mice. Bar: 50 μm.
