TABLE 1.

Comparison of sarcolemma-associated proteins identified from C2C12 cells using the mechanical lysis approach in four independent biological replicates (experiments E1–E4)

Subcellular localization of identified proteins was determined according to a Uniprot Gene Ontology database and literature search. Each protein was designated with only one subcellular location, and the number of proteins in each compartment has been represented as the percentage of total proteins identified.

	E-1	E-2	E-3	E-4	Average
	%	%	%	%	%
Membrane	46	46	47	51	47 ± 2
Secreted	3	5	3	3	3 ± 1
Cytoskeleton	10	10	11	9	10 ± 1
Nucleocytoplasmic	30	25	27	26	27 ± 2
Organelle	10	13	10	9	10 ± 2
Unclassified	2	1	2	2	2 ± 0