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Association of PKCθ with CDCP1. Jurkat-TAg T cells were cotransfected with a FLAG-tagged CDCP1 expression vector plus vectors encoding GFP-tagged wild-type or C2-mutated PKCθ vectors (or empty vector), as indicated. The cells were stimulated with Na3VO4 (1 mm) for 10 min and lysed. Anti-GFP immunoprecipitates (IP) or whole lysates were resolved by SDS-PAGE, transferred to nitrocellulose, and immunoblotted (IB) with the indicated antibodies.
