FIGURE 4.

Crystallographic analysis of rhMIF-p425 complex. A, ribbon diagram depicting the unique binding of the p425 at the interface of two of the tightest packed rhMIF crystallographic trimers. The ligand makes contacts with monomer A (blue) and monomer C (cyan) of one trimer and with monomer B (magenta) of an adjacent trimer. B, ligplot representation of rhMIF-p425 interaction reveals hydrogen bonding (dashed lines) with four residues, Lys³² and Asn¹¹⁰ from one monomer, Glu⁵⁴ from a second monomer, and Ser⁵³ from a monomer of an adjacent trimer. C and D, superimposition of p425-rhMIF complex with HPP-rhMIF and coumarin derivative-rhMIF, respectively. Arrowheads indicate the respective ligands in the active site.