FIGURE 4.

Interaction of AGP with CD14 causes TLR4 activation. dTHP-1 cells were pretreated with anti-CD14 (0.5 μg/ml) antibody (A–C) or anti-TLR4 antibody (1.0 μg/ml) (D–F) for 30 min and were subsequently incubated with AGP (1.0 mg/ml) for 24 h. The levels of expression of IL-6 (A and D), IL-10 (B and E), and CD163 (C and F) were evaluated by quantitative RT-PCR. Results are the means ± S.D. of six separate experiments. Statistically significant reduction was compared with isotype control IgG (**, p < 0.01; *, p < 0.05).