FIGURE 3.

Effects of DIMN on AR and AR target gene expression in prostate cancer cells. A, suppression of AR target gene expression by DIMN in LNCaP and C4–2 cells. Human prostate cancer LNCaP and C4–2 cells maintained in media containing 5% CSS were treated with 10 μm DIMN or BIC in the absence or presence of 10 nm DHT for 48 h prior to harvest. Total RNA was analyzed by Northern blot analysis using cDNA probes for androgen receptor target genes (PSA and NKX3.1). GAPDH expression was used as an internal control. B, suppression of AR and PSA protein expression by DIMN in LNCaP and C4–2 cells. The protein expression levels of AR and PSA were determined by Western blot analysis. The cells maintained as in A were treated with the indicated concentrations of OHF, BIC, or DIMN in the presence of DHT (LNCaP, 10 nm; C4–2, 1 nm) for 48 h prior to harvest. Specific antibodies against the AR and PSA were used for Western blot analysis. Tubulin or GAPDH expression was used as an internal control.