FIGURE 1.

NS5A dimerization occurs directly through Domain I. A and B, GST or GST-Domain I NS5A containing the linker was used as bait to pull down full-length NS5A-His (A) or Domain I NS5A-His (B). Captured proteins were analyzed by Western blotting using anti-His and anti-GST antibodies. C, deletion mutants of Domain I NS5A-His were generated and used as prey in pulldown assays with GST or GST-Domain I NS5A as bait. Captured proteins were analyzed by Western blotting using anti-His and anti-GST antibodies. D, recombinant proteins were treated with benzonase, RNase, or DNase to remove contaminating nucleic acids before pulldown assays. GST-CypA/NS5A-His were used as controls because this interaction has been shown to be direct. Captured proteins were analyzed by Western blotting using anti-His and anti-GST antibodies.