TABLE 5.

Steady-state kinetic parameters of wild-type and mutant AtAPSK

Average values ± S.E. (n = 3) are shown.

Protein	kcat	KmAPS	KiAPS	kcat/Km
	s−1	μm		m−1 s−1 × 108
AtAPSK	272 ± 39	0.48 ± 0.41	37.5 ± 6.9	5.67
AtAPSK D136N	45.0 ± 3.5	0.70 ± 0.29	32.8 ± 7.7	0.64
AtAPSK D136A	29.0 ± 5.1	0.92 ± 0.63		0.31