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Lipid rafts are required for optimal in vivo activity of cytosolic CTA1. HeLa cells were transfected with a plasmid encoding a CTA1 construct that is expressed directly in the cytosol. Intracellular cAMP levels at 2 and 4 h after transfection were then quantified for untreated and filipin-treated cells. The averages ± S.D. (error bars) of 3–4 independent experiments with triplicate samples are shown.
