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OE21 cells incubated with or without 5-aza-2′-deoxycytidine (DAC, 1 or 5 μmol/l) for 96 h, followed by treatment with 0.5 μmol/l trichostatin A (TSA) or vehicle for an additional 24 h. Total RNA was extracted and subjected to the quantitative RT-PCR to measure cellular miR-10a expression levels. Treatment with 1 μmol/l of DAC restored the miR-10a expression levels. TSA treatment did not affect miR-10a expression.
