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Effects of erlotinib in combination with gemcitabine on EGFR signaling in the HPAC pancreatic cancer cell line. HPAC cells were treated with 10 mM erlotinib and/or 100 nM gemcitabine (GEM) for 3 or 7 h. The cells were harvested and immunoblotted for the indicated proteins as described in Materials and methods.
